Zoonotic Diseases

Diseases discussed here have a history of use as an agent for biological warfare, either in the U.S. or abroad. Its use may have been experimental or actual, and any detrimental consequences upon humans, animals or the environment may have been intentional or not, depending on the circumstances, the point in time, and the nature of the disease.

Thursday, September 23, 2010

New Enterobacteriaceae Resistant Isolate Enters U.S.

VERONA INTEGRON-ENCODED METALLO-BETA-LACTAMASE CARRYING

ENTEROBACTERIACEAE - USA ex GREECE: FIRST REPORT

What is Enterobacteriaceae? - http://en.wikipedia.org/wiki/Enterobacteriaceae
More info from CDC; http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5924a5.htm?s_cid=mm5924a5_w
Update: http://www.cdc.gov/mmwr/preview/mmwrhtml/mm59e0921a1.htm?s_cid=mm59e0921a1_w

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Date: Tue 21 Sep 2010

Source: CDC. MMWR Morb Mortal Wkly Rep 2010; 59 (early release); 1-1 [edited]







In July 2010, CDC was notified of a patient with a

carbapenem-resistant _Klebsiella pneumoniae_ strain that produced a

Verona integron-encoded metallo-beta-lactamase (VIM) carbapenemase

(1) not reported previously among _Enterobacteriaceae_ in the USA.

The patient was a woman from the USA who became ill with diarrhea

during a Mediterranean cruise and was hospitalized in Greece, where

she received a diagnosis of sepsis and _Clostridium difficile_ infection.



After 12 days in 2 hospitals in Greece, she was transferred to a

hospital in the USA for continued management of sepsis and acute

renal failure. On admission, blood was drawn for culture through a

central venous catheter that had been placed while the patient was

hospitalized in Greece. The blood subsequently grew

carbapenemase-producing _Klebsiella pneumoniae_ exhibiting the VIM

resistance mechanism, which has been described previously in Greece

but not in the USA. Further testing showed the isolate to be non

susceptible to all antimicrobials usually used to treat _Klebsiella_.

Despite the resistance of the _Klebsiella_ strain, the patient

recovered sufficiently to be discharged after 26 days in the American

hospital. A search for other patients colonized with the same isolate

was conducted by screening 22 patients whose hospital stays

overlapped with this patient; no carbapenem-resistant

_Enterobacteriaceae_ (CRE) were detected.



This report of a VIM-producing CRE follows a June 2010 report of 3

cases of New Delhi metallo-beta-lactamase (NDM-1)-producing

_Enterobacteriaceae_ (2). However, the most common mechanism of

carbapenem resistance among _Enterobacteriaceae_ in the USA remains

the production of the _Klebsiella pneumoniae_ carbapenemase (KPC).

KPC-producing _Enterobacteriaceae_ are widespread in the USA and

other countries (3). Cases of CRE are a significant, emerging public

health problem regardless of the mechanism of carbapenem resistance,

and procedures to rapidly recognize and report CRE cases to infection

prevention personnel should be in place in all acute and

long-term-care facilities. Facilities that have not identified cases

of CRE should undertake periodic laboratory reviews to identify

cases. Patients with CRE should be managed using contact precautions,

and patients exposed to CRE patients (such as roommates) should be

screened with surveillance cultures (3). State and local health

departments should promote adoption of current prevention guidance

and monitoring of the prevalence of these organisms in their jurisdictions (3).



Public health officials and health-care facility staff can consult

with the Division of Healthcare Quality Promotion at CDC on the best

practices for identifying and preventing transmission of these

organisms (e-mail: ).



References

----------

1. Vatopoulos A: High rates of metallo-beta-lactamase-producing

_Klebsiella pneumoniae_ in Greece -- a review of the current

evidence. Euro Surveill 2008; 13(4): 1-6 [available at

].

2. CDC: Detection of _Enterobacteriaceae_ isolates carrying

metallo-beta-lactamase -- United States, 2010. MMWR 2010; 59(24): 750

[available at

].

3. CDC: Guidance for control of infections with carbapenem-resistant

or carbapenemase-producing _Enterobacteriaceae_ in acute care

facilities. MMWR 2009; 58(10): 256-60 [available at

].

--

Communicated by:

ProMED-mail





[The Verona integron-encoded metallo-beta-lactamase carbapenemases

(VIM-1 and VIM-2) were initially identified in carbapenem-resistant

_Pseudomonas aeruginosa_ in the Mediterranean basin. The VIM-1 gene

was originally cloned from a _P. aeruginosa_ strain from an outbreak

in the ICU of the University Hospital in Verona, Italy, hence the name.



Bacteria containing this enzyme have a very broad substrate range

including the carbapenems and most other beta-lactams. The enzymes

are encoded on mobile gene cassettes which have been inserted into an

integron. The integrons easily spread between organisms, playing a

major role in the spread of antimicrobial resistance in gram-negative bacilli.



This resistance gene is the 3rd to be highlighted in the news over

the past 6 weeks. - Mod.LL]



[see also:

NDM-1 carrying Enterobacteriaceae - worldwide ex India, Pakistan (02)

20100914.3325

NDM-1 carrying Enterobacteriaceae - worldwide ex India, Pakistan 20100817.2853

NDM-1 carrying Enterobacteriaceae - N America, UK ex India 20100815.2812

E. coli ST131 - USA: emerging drug-resistant pathogen worldwide 20100803.2607

Antimicrobial resistance monitoring - USA: 2007 report 20100506.1471

2007

----

E. coli, ESBL - UK 20071106.3614

2004

----

E. coli, ESBL - UK (Shropshire) (02) 20040718.1952

E. coli, ESBL - UK (Shropshire) 20040715.1920

2003

----

Acinetobacter, drug resistant - Iraq: RFI 20030417.0934

2002

----

Gram negative bacilli, ESBL - UK (Scotland) 20020527.4333]

...................................mpp/ll/mj/mpp



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